Fluorescent Cell Counting

The sample is run through a flow cytometer and set to stop after a predetermined number of beads are analyzed.

Fluorescent cell counting.

Wash your cells with pbs. From the results the total fluorescence per cell is then analyzed using excel sheet. Biotek s imaging and microscopy products can count cells using a number of microscopy methods including fluorescence microscopy using fluorometric probes that intercalate with nuclear dna color brightfield for trypan blue based viability measurements and label free cell counting using either phase contrast or high contrast brightfield. Bright field green fluorescent and red fluorescent channels were used.

A rectangular section near the analyzed cell that is the non fluorescent part of the image is selected which can be used to minimize the maximum errors in the fluorescent counting of a cell. If you have a cell counter with fluorescent capabilities like we do there is a way around it without adding additional steps to the protocol. Cell counting using a fluorescent dye. Here is our protocol for cell counting with a twist.

Flow cytometric cell counting utilizes a sample with a known concentration of fluorescent beads. Fluorescent cell counting kit fcck is utilized for the fluorometric detection of living cell numbers. Because the concentration of beads is known the volume of sample analyzed can be easily computed.