Fluorescent In Situ Hybridization Probe Design

Multiplex fluorescence in situ hybridization fish enables you to assay multiple targets and visualize colocalized signals in a single specimen.

Fluorescent in situ hybridization probe design.

Fluorescence in situ hybridization fish is a molecular cytogenetic technique that uses fluorescent probes that bind to only those parts of a nucleic acid sequence with a high degree of sequence complementarity it was developed by biomedical researchers in the early 1980s to detect and localize the presence or absence of specific dna sequences on chromosomes. Using spectrally distinct fluorophore labels for each hybridization probe this approach gives you the power to resolve several genetic elements or multiple gene expression patterns through multicolor visual display. The dna probe and the target dna to which the probe will be hybridized. Fluorescence in situ hybridization fish involves the preparation of two main components.

However the well established concepts for oligonucleotide probe design cannot be transferred to polynucleotides. Where the insert may contain a specific gene or originate from a specific chromosomal. The dna probe typically comes from cloned sources such as plasmids cosmids pacs yacs or bacs. Curr opin biotechnol.

Due to the high allele diversity of genes a single probe is not sufficient to detect all alleles of a gene. Wagner m haider s. Fluorescence in situ hybridization fish is a technique that uses fluorescent probes which bind to special sites of the chromosome with a high degree of sequence complementarity to the probes.